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KMID : 0378019760190020187
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New Medical Journal
1976 Volume.19 No. 2 p.187 ~ p.192
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Effect of Steroid Hormone on the Preimplantation Mouse Embryo in Vitro
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Abstract
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The experiments were undertaken in order to study the effect of various steroid hormones on germinal vesicle break-down of mouse oocytes and to determine if there was a different sensitivity of various aged preimplantation mouse embryos to progesterone. Follicular oocytes were obtained from ovaries of A-strain female mouse and early embryos were flushed from their fallopian tubes after an appropriate time of mating. The incubation medium was a modified Krebs-Ringer bicarbonate sol. supplemented with 0.4% bovine serum alumin (BSA). A microtube culture method (Cho, 1974) for the oocytes and embryos were adopted to avoid contamination of steroid hormones which are soluble in oil. Ten to twenty oocytes or embryos were placed in the culture medium in microtube and cultured at 37°C, gassed with 5% CO2 in air humidified in an incubator. After culture, the oocytes or embryos were observed directly through the microtube under an inverted phase microscope. The results from the experiments were as follows.
1. The activity of the steroid hormones on germinal vesicle break-kown of mouse oocytes was dependent upon specificity of the hormones.
2. Oocyte was very resistant to progesterone in germinal vesicle break-down whereas fertilized ova was very sensitive to that by blocking the development of the embryos, from 1-cell to 2-cell stage.
3. When 1-cell, 2-cell, 8-16 cell embryos were cultured for 24 hours in medium containing progesterone, 8-16 cell embryos were more resistant to progesterone than the others in cleavage inhibition. 4. When the embryos were exposed to progesterone long, they became more sensitive to the hormone.
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